1) single molecule detection

2) synthesis of functional nanomaterials for biomedical applications

3) nanophotonics

Full Professor Nankai University, Nankai, Tianjin, China 2016.04-now

Full Professor Hunan Normal University, Changsha, Hunan, China 2013.01-2016-03

Assistant Professor Hunan Normal University, Changsha, Hunan, China 2012.09-2013.01

Postdoc. Research University of Washington, Seattle, WA, U.S.A. 2011.12-2012.09

Postdoc. Research Ames Lab., DOE, Ames, Iowa, U.S.A. 2011.09-2011.12

EDUCATION

Ph. D. Hunan University&Iowa State University 2008-2011 Analytical Chemistry

M. S. Hunan University&HongKong Baptist University 2006-2008 Analytical Chemistry

B. S. Hunan University 2002-2006 Chemistry

59 Xu, Y.; Li, Y.; Wei, L.; Liu, H.; Qiu, J.; Xiao, L.*, Attenuation of the Aggregation and Neurotoxicity of Amyloid Peptides with Neurotransmitter-Functionalized Ultra-Small-Sized Gold Nanoparticles. Eng. Sci., 2019, DOI: 10.30919/es8d778.

58 Zhao, Y.; Cai, J.; Liu, Z.; Li, Y.; Zheng, C.; Zheng, Y.; Chen, Q.; Chen, H.; Ma, F.; An, Y.; Xiao, L.; Jiang, C.; Shi, L.*; Kang, C.*; Liu, Y.*, Nanocomposites Inhibit the Formation, Mitigate the Neurotoxicity, and Facilitate the Removal of beta-Amyloid Aggregates in Alzheimer’s Disease Mice. Nano Lett. 2019, 19, 674-683.

57 Ye, Z.; Liu, H.; Wang, F.; Wang, X.; Wei, L.; Xiao, L.*, Single-particle tracking discloses binding-mediated rocking diffusion of rod-shaped biological particles on lipid membranes. Chem. Sci. 2019, 10, 1351-1359.

56 Ma, Y.; Wang, X.; Liu, H.; Wei, L.; Xiao, L.*, Recent advances in optical microscopic methods for single-particle tracking in biological samples. Anal. Bioanal. Chem. 2019. https://doi.org/10.1007/s00216-019-01638-z.

55 Liu, H.; Ye, Z.; Wang, X.; Wei, L.; Xiao, L.*, Molecular and living cell dynamic assays with optical microscopy imaging techniques. Analyst 2019, 144, 859-871.

***********************************2018***********************************

54 Ye, Z.; Liu H.; Xiao L.* Recent Advances of Single Molecule/Particle Imaging with Optical Microscopic Methods. Sci. Sin. Chem. 2018, 48, https://doi.org/10.1360/N032018-00199. (Review, 庆祝南开大学成立100周年专刊)

53 Zhang, P.; Wang, H.; Zhang, D.; Zeng, X.; Zeng, R.*; Xiao, L.*; Tao, H.; Long, Y.; Yi, P.; Chen, J.*, Two-photon fluorescent probe for lysosome-targetable hypochlorous acid detection within living cells. Sens. Actuators B Chem. 2018, 255, 2223-2231.

52 Zhang, D.; Wei, L.; Zhong, M.; Xiao, L.*; Li, H.-W.; Wang, J., The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy. Chem. Sci. 2018, 9, 5260-5269.

51 Yu, Y.; Ma, L.; Li, L.; Deng, Y.; Xu, L.; Liu, H.; Xiao, L.*; Su, X.*, Digestion of Dynamic Substrate by Exonuclease Reveals High Single-Mismatch Selectivity. Anal. Chem. 2018, 90, 13655-13662.

50 Ye, Z.; Weng, R.; Ma, Y.; Wang, F.; Liu, H.; Wei, L.*; Xiao, L.*, Label-Free, Single-Particle, Colorimetric Detection of Permanganate by GNPs@Ag Core-Shell Nanoparticles with Dark-Field Optical Microscopy. Anal. Chem. 2018, 90, 13044-13050.

49 Ye, Z.; Wei, L.; Zeng, X.; Weng, R.; Shi, X.; Wang, N.; Chen, L.; Xiao, L.*, Background-Free Imaging of a Viral Capsid Proteins Coated Anisotropic Nanoparticle on a Living Cell Membrane with Dark-Field Optical Microscopy. Anal. Chem. 2018, 90, 1177-1185.

48 Wei, L.; Zhang, D.; Zheng, X.; Zeng, X.; Zeng, Y.; Shi, X.; Su, X.; Xiao, L.*, Fabrication of Positively Charged Fluorescent Polymer Nanoparticles for Cell Imaging and Gene Delivery. Nanotheranostics 2018, 2, 157-167.

47 Wang, Y.-M.; Yang, Z.-R.; Xiao, L.; Yin, X.-B.*, Lab-on-MOFs: Color-Coded Multitarget Fluorescence Detection with White-Light Emitting Metal Organic Frameworks under Single Wavelength Excitation. Anal. Chem. 2018, 90, 5758-5763.

46 Shi, X.*; He, Y.; Gao, W.; Liu, X.; Ye, Z.; Liu, H.; Xiao, L.*, Quantifying the Degree of Aggregation from Fluorescent Dye-Conjugated DNA Probe by Single Molecule Photobleaching Technology for the Ultrasensitive Detection of Adenosine. Anal. Chem. 2018, 90, 3661-3665.

45 Russel, M.*; Sophocleous, M.; Shan, J.; Xu, W.; Xiao, L.; Maskow, T.; Alam, M.; Georgiou, J., High-frequency, dielectric spectroscopy for the detection of electrophysiological/biophysical differences in different bacteria types and concentrations. Anal. Chim. Acta 2018, 1028, 86-95.

44 Qi, F.; Han, Y.; Ye, Z.; Liu, H.; Wei, L.; Xiao, L.*, Color-Coded Single-Particle Pyrophosphate Assay with Dark-Field Optical Microscopy. Anal. Chem. 2018, 90, 11146-11153.

43 Pan, A.; Zhong, M.; Wu, H.; Peng, Y.; Xia, H.; Tang, Q.; Huang, Q.; Wei, L.; Xiao, L.*; Peng, C.*, Topical Application of Keratinocyte Growth Factor Conjugated Gold Nanoparticles Accelerate Wound Healing. Nanomedicine: NBM 2018, 14, 1619-1628.

42 Li, X.; Wei, L.; Pan, L.; Yi, Z.; Wang, X.; Ye, Z.; Xiao, L.*; Li, H.-W.*; Wang, J., Homogeneous Immunosorbent Assay Based on Single-Particle Enumeration Using Upconversion Nanoparticles for the Sensitive Detection of Cancer Biomarkers. Anal. Chem. 2018, 90, 4807-4814.

41 Deng, M.; Tang, Y.; Li, W.; Wang, X.; Zhang, R.; Zhang, X.; Zhao, X.; Liu, J.; Tang, C.; Liu, Z.; Huang, Y.; Peng, H.; Xiao, L.; Tang, D.; Scott, M. J.; Wang, Q.; Liu, J.; Xiao, X.; Watkins, S.; Li, J.; Yang, H.; Wang, H.; Chen, F.; Tracey, K. J.; Billiar, T. R.*; Lu, B.*, The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis. Immunity 2018, 49, 740-753.

***********************************2017***********************************

40 Zhong, W.; Zeng, X.; Chen, J.*; Hong, Y.; Xiao, L.; Zhang, P.*, Photoswitchable fluorescent polymeric nanoparticles for rewritable fluorescence patterning and intracellular dual-color imaging with AIE-based fluorogens as FRET donors. Polym. Chem. 2017, 8, 4849-4855.

39 Zhang, Z.; Zhang, D.; Wei, L.; Wang, X.; Xu, Y.; Li, H.-W.; Ma, M.*; Chen, B.; Xiao, L.*, Temperature responsive fluorescent polymer nanoparticles (TRFNPs) for cellular imaging and controlled releasing of drug to living cells. Colloid. Surface. B. 2017, 159, 905-912.

38 Wei, L.; Ma, Y.; Zhu, X.; Xu, J.; Wang, Y.; Duan, H.; Xiao, L.*, Sub-diffraction-limit localization imaging of a plasmonic nanoparticle pair with wavelength resolved dark-field microscopy. Nanoscale 2017, 9, 8747-8755.

37 Wei, L.; Ma, Y.; Shi, X.; Wang, Y.; Su, X.; Yu, C.; Xiang, S.; Xiao, L.*; Chen, B.*, Living cell intracellular temperature imaging with biocompatible dye-conjugated carbon dots. J. Mater. Chem. B 2017, 5, 3383-3390.

36 Wang, N.; Zhang, Y.; Lei, X.; Yu, W.; Zhan, Y.; Wang, D.; Zhang, J.; Wang, A.; Xiao, L.; Jiang, P.*; Yang, Y.*, Optimized conditions for preserving stability and integrity of porcine circovirus type2 virus-like particles during long-term storage. J. Virol. Methods 2017, 243, 146-150.

35 Wang, J.; Xiong, G.; Ma, L.; Wang, S.; Zhou, X.; Wang, L.; Xiao, L.; Su, X.*; Yu, C.*, A dynamic sandwich assay on magnetic beads for selective detection of single-nucleotide mutations at room temperature. Biosens. Bioelectron. 2017, 94, 305-311.

34 Tan, H.; Huang, Y.; Xu, J.; Chen, B.; Zhang, P.; Ye, Z.; Liang, S.; Xiao, L.*; Liu, Z.*, Spider Toxin Peptide Lycosin-I Functionalized Gold Nanoparticles for in vivo Tumor Targeting and Therapy. Theranostics 2017, 7, 3168-3178.

33 Su, X.; Li, Z.; Yan, X.; Wang, L.; Zhou, X.; Wei, L.; Xiao, L.*; Yu, C.*, Telomerase Activity Detection with Amplification-Free Single Molecule Stochastic Binding Assay. Anal. Chem. 2017, 89, 3576-3582.

32 Song, W.; Duan, W.; Liu, Y.; Ye, Z.; Chen, Y.; Chen, H.; Qi, S.; Wu, J.; Liu, D.; Xiao, L.*; Ren, C.*; Chen, X., Ratiometric Detection of Intracellular Lysine and pH with One-Pot Synthesized Dual Emissive Carbon Dots. Anal. Chem. 2017, 89, 13626-13633.

***********************************2016***********************************

31 Yang, Q.; Ye, Z.; Zhong, M.; Chen, B.; Chen, J.; Zeng, R.; Wei, L.*; Li, H.-W.*; Xiao, L.*, Self-Assembled Fluorescent Bovine Serum Albumin Nanoprobes for Ratiometric pH Measurement inside Living Cells. ACS Appl. Mater. Interfaces 2016, 8, 9629-9634.

30 Wei, L.; Ye, Z.; Xu, Y.; Chen, B.; Yeung, E. S.; Xiao, L.*, Single Particle Tracking of Peptides-Modified Nanocargo on Lipid Membrane Revealing Bulk-Mediated Diffusion. Anal. Chem. 2016, 88, 11973-11977.

29 Wei, L.; Ye, Z.; Luo, W.; Chen, B.; Xiao, L.*, Redistribution of fluorescent molecules at the solid/liquid interface with total internal reflection illumination. Talanta 2016, 155, 229-234.

28 Wei, L.; Xu, J.; Ye, Z.; Zhu, X.; Zhong, M.; Luo, W.; Chen, B.; Duan, H.; Liu, Q.; Xiao, L.*, Orientational Imaging of a Single Gold Nanorod at the Liquid/Solid Interface with Polarized Evanescent Field Illumination. Anal. Chem. 2016, 88, 1995-1999.

27 Tan, H.; Luo, W.; Wei, L.; Chen, B.; Li, W.; Xiao, L.*; Manzhos, S.*; Liu, Z.*; Liang, S., Quantifying the Distribution of the Stoichiometric Composition of Anticancer Peptide Lycosin-I on the Lipid Membrane with Single Molecule Spectroscopy. J. Phys. Chem. B 2016, 120, 3081-3088.

26 Poon, C.-Y.; Wei, L.; Xu, Y.; Chen, B.; Xiao, L.*; Li, H.-W.*, Quantification of Cancer Biomarkers in Serum Using Scattering-Based Quantitative Single Particle Intensity Measurement with a Dark-Field Microscope. Anal. Chem. 2016, 88, 8849-8856.

25 Ma, Y.; Zhang, Z.; Xu, Y.; Ma, M.; Chen, B.; Wei, L.*; Xiao, L.*, A bright carbon-dot-based fluorescent probe for selective and sensitive detection of mercury ions. Talanta 2016, 161, 476-481.

24 Luo, W.; Wu, M.; Li, S.; Xu, Y.; Ye, Z.; Wei, L.; Chen, B.; Xu, Q.-H.; Xiao, L.*, Nanoprecipitation of Fluorescent Conjugated Polymer onto the Surface of Plasmonic Nanoparticle for Fluorescence/Dark-Field Dual-Modality Single Particle Imaging. Anal. Chem. 2016, 88, 6827-6835.

***********************************2015***********************************

23 Yang, Q.; Wei, L.; Zheng, X.; Xiao, L.*, Single Particle Dynamic Imaging and Fe3+ Sensing with Bright Carbon Dots Derived from Bovine Serum Albumin Proteins. Sci. Rep. 2015, 5.

***********************************2014***********************************

22 Xiao, L.*; Yeung, E. S.*, Optical Imaging of Individual Plasmonic Nanoparticles in Biological Samples. Ann. Rev. Anal. Chem. 2014, 7, 89-111.

21 Wei, L.; Zhou, P.; Yang, Q.; Yang, Q.; Ma, M.; Chen, B.; Xiao, L.*, Fabrication of bright and small size semiconducting polymer nanoparticles for cellular labelling and single particle tracking. Nanoscale 2014, 6, 11351-11358.

20 Wei, L.; Yang, Q.; Xiao, L.*, Tempo-spatially resolved cellular dynamics of human immunodeficiency virus transacting activator of transcription (Tat) peptide-modified nanocargos in living cells. Nanoscale 2014, 6, 10207-10215.

19 Li, H.; Guo, Y.; Xiao, L.*; Chen, B.*, Selective and sensitive detection of acetylcholinesterase activity using denatured protein-protected gold nanoclusters as a label-free probe. Analyst 2014, 139, 285-289.

18 Li, H.; Guo, Y.; Xiao, L.*; Chen, B.*, A fluorometric biosensor based on H2O2-sensitive nanoclusters for the detection of acetylcholine. Biosens. Bioelectron. 2014, 59, 289-292.

***********************************2013***********************************

17 Xiong, X.; Yang, Z.; Li, Y.; Xiao, L.; Jiang, L.; Chen, Y.; Ma, M.; Chen, B.*, Preparation of a polyhedral oligomeric silsesquioxane-based perfluorinated monolithic column. J. Chromatogr. A. 2013, 1304, 85-91.

16 Wei, L.; Zhao, X.; Chen, B.; Li, H.; Xiao, L.*; Yeung, E. S.*, Frozen Translational and Rotational Motion of Human Immunodeficiency Virus Transacting Activator of Transcription Peptide-Modified Nanocargo on Neutral Lipid Bilayer. Anal. Chem. 2013, 85, 5169-5175.

15 Wei, L.; Liu, C.; Chen, B.; Zhou, P.; Li, H.; Xiao, L.*; Yeung, E. S.*, Probing Single-Molecule Fluorescence Spectral Modulation within Individual Hotspots with Subdiffraction-Limit Image Resolution. Anal. Chem. 2013, 85, 3789-3793.

14 Sun, W.; Xiao, L.; Fang, N.*, Imaging non-fluorescent nanoparticles in living cells with wavelength-dependent differential interference contrast microscopy and planar illumination microscopy. Methods Mol. Biol. 2013, 931, 169-186.

13 Li, Y.; Xiao, L.-H.; Zhou, N.-Y.; Yuan, P.; Wu, Y.-N.*; Chen, B.*, Purification of Pigments by Iron Oxide Nanoparticles for Analysis of Pesticide Residues in Tea. Chinese J. Anal. Chem. 2013, 41, 63-68.

+++++++++++++++++++++++++Independent Work+++++++++++++++++++++++++++

***********************************2012***********************************

12 Xiao, L.; Wei, L.; Liu, C.; He, Y.; Yeung, E. S.*, Unsynchronized Translational and Rotational Diffusion of Nanocargo on a Living Cell Membrane. Angew. Chem. Int. Ed. 2012, 51, 4181-4184.

11 Xiao, L.; Ha, J. W.; Wei, L.; Wang, G.; Fang, N.*, Determining the Full Three-Dimensional Orientation of Single Anisotropic Nanoparticles by Differential Interference Contrast Microscopy. Angew. Chem. Int. Ed. 2012, 51, 7734-7738.

10 Chan, H.-M.; Xiao, L.; Yeung, K.-M.; Ho, S.-L.; Zhao, D.; Chan, W.-H.; Li, H.-W.*, Effect of surface-functionalized nanoparticles on the elongation phase of beta-amyloid (1-40) fibrillogenesis. Biomaterials 2012, 33, 4443-4450.

***********************************2011***********************************

9 Xiao, L.; Wei, L.; Cheng, X.; He, Y.*; Yeung, E. S., Noise-Free Dual-Wavelength Difference Imaging of Plasmonic Resonant Nanoparticles in Living Cells. Anal. Chem. 2011, 83, 7340-7347.

8 Xiao, L.; Qiao, Y.; He, Y.*; Yeung, E. S., Imaging Translational and Rotational Diffusion of Single Anisotropic Nanoparticles with Planar Illumination Microscopy. J. Am. Chem. Soc. 2011, 133, 10638-10645.

7 Wei, L.; Xiao, L.; He, Y.*, Synthesis of water soluble silver-nanoparticle-embedded polymer nanofibers with poly(2-ethyl-2-oxazoline) by a straightforward polyol process. J. Mater. Res. 2011, 26, 1614-1620.

6 Sun, W.; Xiao, L.; Fang, N. Cell Imaging Techniques: Methods and Protocols, Methods in Molecular Biology, Human Press. Springer, Book Chapter, 2011.

***********************************2010***********************************

5 Xiao, L.; Zhao, D.; Chan, W.-H.; Choi, M. M. F.; Li, H.-W.*, Inhibition of beta 1-40 amyloid fibrillation with N-acetyl-L-cysteine capped quantum dots. Biomaterials 2010, 31, 91-98.

4 Xiao, L.; Wei, L.; He, Y.*; Yeung, E. S., Single Molecule Biosensing Using Color Coded Plasmon Resonant Metal Nanoparticles. Anal. Chem. 2010, 82, 6308-6314.

3 Xiao, L.; Qiao, Y.; He, Y.*; Yeung, E. S.*, Three Dimensional Orientational Imaging of Nanoparticles with Darkfield Microscopy. Anal. Chem. 2010, 82, 5268-5274.

***********************************2009***********************************

2 Xiao, L.; Zhou, R.; He, Y.*; Li, Y.; Yeung, E. S.*, Direct Observation of Nanoparticle Self-Assembly Dynamics at the Water-Air Interface Using Differential Interference Contrast Microscopy. J. Phys. Chem. C 2009, 113, 1209-1216.

1 Xiao, L.; He, Y.*; Yeung, E. S.*, High Throughput Single Molecule Spectral Imaging of Photoactivated Luminescent Silver Clusters on Silver Island Films. J. Phys. Chem. C 2009, 113, 5991-5997.