It is desirable to develop electrode materials for advanced rechargeable batteries with low cost, long life, and high-rate capability. Pyrite FeS₂, as an easily obtained natural mineral, has been already commercialized in primary lithium batteries, but encountered problems in rechargeable batteries with carbonate-based electrolytes due to the limited cycle life caused by the conversion-type reaction (FeS₂ + 4M → Fe + 2M₂S (M = Li or Na)). Herein, we demonstrate that FeS₂ microspheres can be applied in room-temperature rechargeable sodium batteries with only the intercalation reaction by simultaneously selecting a compatible NaSO₃CF₃/diglyme electrolyte and tuning the cut-off voltage to 0.8 V. A surprisingly high-rate capability (170 mA h g⁻¹ at 20 A g⁻¹) and unprecedented long-term cyclability (∼90% capacity retention for 20 000 cycles) has been obtained. We suggest that a stable electrically conductive layer-structured Na_xFeS₂ was formed during cycling, which enables the highly reversible sodium intercalation and deintercalation. Moreover, 18650-type sodium batteries were constructed exhibiting a high capacity of ∼4200 mA h (corresponding to 126 W h kg⁻¹ and 382 W h L⁻¹) and a capacity retention of 97% after an initial 200 cycles at 4 A during charge–discharge. This shows that the production of rechargeable sodium batteries with FeS₂ microspheres is viable for commercial utilization.