黄笛

黄笛，副研究员，硕士生导师。研究方向：功能寡糖和糖蛋白合成生物学。

2008年毕业于大连理工大学，获学士学位，2010年获天津大学硕士学位，2013年获天津大学博士学位，导师为闻建平教授。2013年7月进入南开大学工作，现任南开大学泰达生物技术研究院和南开国际先进研究院（深圳福田）副研究员；主要研究领域为微生物母乳寡糖、糖蛋白等产物的合成生物学，长期致力于母乳寡糖、糖蛋白的生物合成等方面的系统研究，重点开发系统生物学、合成生物学技术与功能基因组技术在糖化学中的理论基础及应用，整合多组学数据，解析微生物的生理特性和调控机制，辅以计算机设计开发创建新型原核生物细胞工厂，实现工业化菌株的个性化定制。

以第一或通讯作者在Metabolic Engineering、Green Chemistry、Carbohydrate Polymers、Biotechnology and Bioengineering、mSystems、Biotechnology for Biofuels、International Journal of Food Microbiology、Bioresource Technology、Applied Microbiology and Biotechnology、Microbial Cell Factories等生物期刊上发表30余篇SCI论文。

主持3项国家自然科学基金、2项天津市自然科学基金项目、3项中央高校基本科研业务费，作为学术课题骨干参与完成了973项目、国家自然科学基金重点（国际合作）项目等多个项目课题。2017年入选天津市“131”第三层次人才。

联系电话：022-65333286

Email：huangdi@nankai.edu.cn

部分代表作

第一和通讯作者（含共同）

1.       Xiaolong Jiang, Jing Bai, Huijing Zhang, Jian Yuan, Gege Lu, Yuhui Wang, Lingyan Jiang, Bin Liu, Di Huang^*, Lu Feng^*. Development of an O-polysaccharide based recombinant glycoconjugate vaccine in engineered E. coli against ExPEC O1. Carbohydrate Polymers, 2022, 277:118796.

2.       Lu Wang, Wenxuan Zhu, Gege Lu, Pan Wu, Yi Wei, Yingying Su, Tianyuan Jia, Linxing Li, Xi Guo, Min Huang, Qian Yang, Di Huang^*, Bin Liu^*. In silico species identification and serotyping for Cronobacter isolates by use of whole-genome sequencing data. International Journal of Food Microbiology, 2021. 358:109405.

3.       Di Huang#, Yuhui Wang#, Jing Zhang#, Huimin Xu, Jing Bai, Huijing Zhang, Xiaolong Jiang, Jian Yuan, Gege Lu, Lingyan Jiang, Xiaoping Liao, Bin Liu, Huanhuan Liu. Integrative metabolomic and transcriptomic analyses uncover metabolic alterations and pigment diversity in Monascus in response to different nitrogen sources. mSystems, 2021.6(5):e0080721.

4.       Huanhuan Liu, Jing Zhang, Gege Lu, Fanghui Wang, Lin Shu, Huimin Xu, Zhenjing Li, Yurong Wang, Qingbin Guo, Shufen Wu, Lingyan Jiang, Changlu Wang^*, Di Huang^*, Bin Liu^*. Comparative metabolomics analysis reveals the metabolic regulation mechanism of yellow pigment overproduction by Monascus using ammonium chloride as a nitrogen source. Applied Microbiology and Biotechnology, 2021.105(16-17):6369-6379.

5.       Jian Yuan^#, Xiaolong Jiang^#, Huijing Zhang, Jing Bai, Jing Zhang, Yuhui Wang, Gege Lu, Huimin Xu, Huanhuan Liu^*, Bin Liu^*, Di Huang^*. Construction and optimization of a microbial platform for sustainable biosynthesis of poly-N-acetyllactosamine glycoprotein in the cytoplasm for detecting tumor biomarker galectin-3. Green Chemistry, 2021, 23:2668–2684. (HighlightasBackcoverarticle)

6.       Xiaolong Jiang, Jing Bai, Jian Yuan, Huijing Zhang, Gege Lu, Yuhui Wang, Lingyan Jiang, Bin Liu, Lei Wang, Di Huang^*, Lu Feng^*. High efficiency biosynthesis of O-polysaccharide-based vaccines against extraintestinal pathogenic Escherichia coli. Carbohydrate Polymers, 2021, 255:117475.

7.       Huanhuan Liu, Jing Zhang, Jian Yuan, Xiaolong Jiang, Lingyan Jiang, Zhenjing Li, Zhiqiu Yin, Yuhui Du, Guang Zhao, Bin Liu^*, Di Huang^*. Gene coexpression network analysis reveals a novel metabolic mechanism of Clostridium acetobutylicum responding to phenolic inhibitors from lignocellulosic hydrolysates. Biotechnology for Biofuels, 2020, 13:163.

8.       Dalong Hu^#, Zhiqiu Yin^#, Chao Yuan^#, Pan Yang, Chengqian Qian, Yi Wei, Si Zhang, Yuhui Wang, Jian Yuan, Meng Wang, Peter R. Reeves, Lihong Tu, Min Chen^*, Di Huang^*, Bin Liu^*. Changing molecular epidemiology of Vibrio cholerae outbreaks in Shanghai, China. mSystems, 2019, 4(6). pii: e00561-19.

9.       Zhiqiu Yin^#, Chao Yuan^#, Yuhui Du^#, Pang Yang, Chengqian Qian, Yi Wei, Si Zhang, Di Huang^*, Bin Liu^*. Comparative genomic analysis of the Hafnia genus reveals an explicit evolutionary relationship between the species alvei and paralvei and provides insights into pathogenicity. BMC Genomics, 2019, 20(1):768.

10.     Cheng Wang^*, Junhua Wang, Jian Yuan, Lingyan Jiang, Xiaolong Jiang, Bin Yang, Guang Zhao, Bin Liu, Di Huang^*. Generation of Streptomyces hygroscopicus cell factories with enhanced ascomycin production by combined elicitation and pathway-engineering strategies. Biotechnology and Bioengineering, 2019, 116(12):3382-3395.

11.     Huanhuan Liu, Jing Zhang, Jian Yuan, Xiaolong Jiang, Lingyan Jiang, Guang Zhao, Di Huang^*, Bin Liu^*. Omics‑based analyses revealed metabolic responses of Clostridium acetobutylicum to lignocellulose‑derived inhibitors furfural, formic acid and phenol stress for butanol fermentation. Biotechnology for Biofuels, 2019, 12:101.

12.     Linbo Sun^#, Di Huang^#, Lin Zhu^#, Bingling Zhang, Chenchen Peng, Ting Ma, Xin Deng, Junli Wu, Wei Wang^*. Novel thermostable enzymes from Geobacillus thermoglucosidasius W-2 for high-efficient nitroalkane removal under aerobic and anaerobic conditions. Bioresource Technology, 2019, 278:73-81.

13.     Chenchen Peng^#, Di Huang^#, Yukun Shi, Bingling Zhang, Linbo Sun, Mingchang Li, Xin Deng, Wei Wang^*.Comparative transcriptomic analysis revealed the key pathways responsible for organic sulfur removal by thermophilic bacterium Geobacillus thermoglucosidasius W-2. Science of the Total Environment, 2019, 676: 639–650.

14.     Di Huang, Kexin Yang, Jia Liu, Yingying Xu, Yuanyuan Wang, Ru Wang, Bin Liu^*, Lu Feng^*, Metabolic engineering of Escherichia coli for the production of 2′-fucosyllactose and 3-fucosyllactose through modular pathway enhancement. Metabolic Engineering, 2017, 41:23-38.

15.     Di Huang^*, Ru Wang, Wenjie Du, Guanyi Wang, Menglei Xia, Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol. Bioresource Technology, 2015, 196:263-272.

16.     Di Huang, Jia Liu, Yanfei Qi, Kexin Yang, Yingying Xu, Lu Feng^*, Synergistic hydrolysis of xylan using novel xylanases, β-xylosidases, and an α-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Applied Microbiology and Biotechnology, 2017, 101(15):6023-6037.

17.     Huanhuan Liu^#, Di Huang^#, Lina Jin^#, Cheng Wang, Shaoxiong Liang, Jianping Wen^*, Integrating multi-omics analyses of Nonomuraea dietziae to reveal the role of soybean oil in [(4′-OH)MeLeu]⁴-CsA overproduction. Microbial Cell Factories, 2017, 16:120.

18.     Huanhuan Liu^#, Di Huang^#, Jianping Wen^*, Integrated intracellular metabolic profiling and pathway analysis approaches reveal complex metabolic regulation by Clostridium acetobutylicum. Microbial Cell Factories, 2016, 15:36.

19.     Guanyi Wang^#, Di Huang^#, Yong Li, Jianping Wen^*, Xiaoqiang Jia, A metabolic-based approach to improve xylose utilization for fumaric acid production from acid pretreated wheat bran by Rhizopus oryzae. Bioresource Technology, 2015, 180:119-127.

20.     Jun Wang^#, Huanhuan Liu^#, Di Huang^#, Lina Jin, Cheng Wang, Jianping Wen^*, Comparative proteomic and metabolomic analysis of Streptomyces tsukubaensis reveals the metabolic mechanism of FK506 overproduction by feeding soybean oil. Applied Microbiology and Biotechnology, 2017, 101(6):2447-2465.

21.     Cheng Wang^*#, Di Huang^#, Shaoxiong Liang. Identification and metabolomic analysis of chemical elicitors for tacrolimus accumulation in Streptomyces tsukubaensis. Applied Microbiology and Biotechnology, 2018, 102:7541-7553.

22.     Di Huang, Shanshan Li, Menglei Xia, Jianping Wen^*, Xiaoqiang Jia. Genome-scale metabolic network guided engineering of Streptomyces tsukubaensis for FK506 production improvement. Microbial Cell Factories, 2013, 12:52.

23.     Di Huang, Jianping Wen^*, Guoying Wang, Guanghai Yu, Xiaoqiang Jia and Yunlin Chen. In silico aided metabolic engineering of Streptomyces roseosporus for daptomycin yield improvement. Applied Microbiology and Biotechnology, 2012, 94(3):637-649.

24.     Guanyi Wang^#, Di Huang^#, Haishan Qi, Jianping Wen^*, Xiaoqiang Jia. Rational medium optimization based on comparative metabolic profiling analysis to improve fumaric acid production. Bioresource Technology, 2013, 137:1-8.

25.     Menglei Xia^#, Di Huang^#, Shanshan Li, Jianping Wen^*, Xiaoqiang Jia, Yunlin Chen. Enhanced FK506 production in Streptomyces tsukubaensis by rational feeding strategies based on comparative metabolic profiling analysis. Biotechnology and Bioengineering, 2013, 110(10):2717-2730.